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Causes sagging of breasts and Bra
Sagging or drooping of breasts is a natural, inevitable process that happens to all females at some point - except those with fairly small breasts. The most notable sagging happens with the process called breast involution, but breasts can start drooping a little at any age, because they do NOT have muscles in them. They have ligaments and connective tissue. When the gravity pulls the breasts down, those ligaments and the skin can stretch, and so the breast then droops. This depends on the elasticity of your skin and of your ligaments, as determined by your genes and diet, and also on normal aging processes. Obviously large breasts will sag easier since the gravity is pulling them down more.
Breast involution is a process where the milk-making system inside the breast shrinks because it's not needed anymore. This happens either after weaning, or right after pregnancy if you don't breastfeed at all, or during menopause. When the tissues inside breast shrink, and the skin surrounding it doesn't, the breast can look 'empty' and saggy. Over time, some fat gets deposited back to the breast so it will look somewhat fuller but sagging usually remains.
Bras will keep your breasts from sagging only temporally, while you're wearing them - but not for the remainder of time. The breast itself is not muscle, so keeping it toned up is impossible. What it can do, particularly for larger-breasted females, is obviously to provide the comfort and the support. So, if a female wants a particular breast profile, she will buy a particular brand, and that is what they're designed for. There's no permanent effect on the breast from wearing a particular bra. The bra will give you the shape the bra's been designed to give while you're wearing it. When you take it off, you go natural.
However, there is some support for the idea that breasts in bras actually sag more (when bra is taken off). How could that be since the main idea of bras is to lift the breasts upward? The main idea is that the ligaments which support breasts in normal circumstances, would atrophy get thinner and weaker, when breasts are immobilized within bras and don't have to bear their own weight.
There are lots of studies showing that ligaments and tendons in limbs do atrophy when the limb is immobilized. Bras obviously prevent the natural slight up-down movement of breasts when we walk, and let shoulders bear the weight of breasts. If breast ligaments behave like limb ligaments, it may be a matter of "use it or lose it". The unspoken rules of the society may force you to wear a bra to work or to social occasions, but there's no need to wear one for the purpose of preventing sagging. It just won't work. Remember also that breasts will benefit from bra-free time, so it is good to take the bra off whenever you can. Large breasts often droop at a young age.
Could sagging be better for breast health?
Fashion magazines and ads may cause you to think that pert, hard breasts are more optimal, but some research suggests that the opposite is true and that saggier breasts might be healthier.